Catabolite Repression and Induction of Beta-galactosidase Synthesis Lab Report

Catabolite Repression and Induction of Beta-galactosidase Synthesis - Lab Report frameworkApart from induction, synthesis rate is determined by catabolite repression, whereby it dims down the synthesis of beta-galactosidase especially in the strawman of a better carbon (and ability) source, such as glucose. In this experiment, Escherichia coli (E. coli) is used as the bacteria to make water synthesis of enzyme -galactosidase. The results support milk sugar metabolism by newly synthesised beta-galactosidase and also, quantitatively, IPTG is a more effective persuader of beta-galactosidase synthesis than lactose.In order to regulate the gene expression levels in a cell, there be certain mechanisms that must be considered in operation. In which case, the dominion is considered at transcription and translation levels or the stability of messenger RNA. The aforementioned can only work in regulation based on the synthesis of a particular protein. Consequently, it comes out as a sub ject of importance to investigate the regulation of transcription of bacterial genes. For this case, Escherichia coli (E. coli) is used as the bacteria to induce synthesis of enzyme -galactosidase.Escherichia coli (E. coli) can produce the enzyme -galactosidase which breaks lactose into galactose and glucose. Synthesis of the enzyme beta-galactosidase is induced in wild-type E. coli strains in response to the presence of lactose, the enzymes natural substrate (Ring, 1999, 80). The inducer, lactose, is ordinarily the molecule broken down by the enzyme system. Worth noting is the ability of E.coli to solely use lactose as a carbon source regardless of the presence of glucose. A more stable inducer that lactose, IPTG (Isopropyl -D-1-thiogalactopyranoside) helps in inducing expression of the enzyme without being metabolized in the process.Apart from induction, synthesis of -galactosidase is also influenced by catabolite repression. The process involves slow down of the synthesis proces s, facilitated by lactose, especially when a presence of glucose is detected. Intuitively, glucose acts as a better energy and carbon source than lactose (Wallenfels, 1972, 67). When given both sugars, E.coli will not synthesize beta-galactosidase until all of the glucose is first base exhausted from the medium.